UV-Visible Detectors
The most commonly used detector for HPLC analysis is the UV-visible detector. The detector fulfills many of the criteria for an ideal detector. This module will detail the workings of UV-visible detectors, including variable wavelength and diode array detectors. Key detectors settings that should be optimized are fully detailed.
Topics include:
- The flow cell
- UV-visible detection - quantitation
- External standard quantitation
- Calibration curve
- UV absorbance
- Variable wavelength UV-visible detectors
- Diode array detectors (DAD)
- Diode array detectors spectra
- Diode array detectors - bandwidth
- Diode array detectors - slit width
- Diode array detectors - response time
- Diode array detectors - reference wavelength
- Choosing sample and reference settings for DAD
- DAD - peak suppression
