COURSE OVERVIEW
Who is this course for?
Users of LC-MS and LC-MS-MS equipment, or anyone interested in learning the analytical capabilities afforded by this type of instrument.
Previous Knowledge
No previous knowledge of mass spectrometry is necessary, but an understanding of the fundamentals of liquid chromatography is beneficial.
What you will learn
- How modern API designs work and which one best fits your LC and application requirements
- How ions are produced and how to optimise LC mobile phase and interface parameters to achieve the highest sensitivity, accuracy and reproducibility
- The anatomy and working principles of quadrupoles, ion traps, time-of-flight detectors and more
- The information that can be acquired in MS, MS-MS and MSn modes, and how to optimise instrument settings for high data quality
COURSE OUTLINE
Application Areas of LC-MS
- Drug discovery & metabolite identification
- Environmental analysis & food safety
- Proteomics & biomarker research
Troubleshooting Common LC-MS Issues
- Developing robust LC-MS methods
- Validation parameters (sensitivity, precision, accuracy, linearity)
- Regulatory considerations (FDA, EMA, ICH guidelines)
Q&A and Closing Discussion
- Summary of Key Concepts
- Open Discussion on Challenges Faced in LC-MS
- Resources for Further Learning